(DDC)2Zn, disulfiram (DS) metabolite, has shown promising anticancer effects in vitro but further investigations in vivo are limited by its poor water solubility. In this study, liposomes are assessed as a delivery system for (DDC)2Zn. Liposomes were prepared by the thin-film hydration method, followed by high-pressure homogenisation (HPH) for size reduction. The nano-liposomes were then characterised by size, polydispersity index (PDI), zeta potential (ZP), drug loading and encapsulation efficiencies(DLE% and EE%), and MTT cytotoxicity assay. The HSPC-based (PBS) liposomes showed a nano-range of sizes (< 200nm), good PDI (<0.5) but moderate EE%(<40%). However, (DDC)2Zn liposomal formulations showed enhanced cytotoxic activities toward colorectal cancer cells. Therefore, liposomal formulations of(DDC)2Zn with improved DLE% and EE% might have immense potential in cancer therapy.
Disulfiram, Zinc, Colorectal cancer, Liposomes, diethyldithiocarbamate
How to Cite
Ansari-Fard, N. & Kaya, A. & Alves, D. & Arafat, B. & Pierscionek, B. & Najlah, M., (2022) “Development of Liposomal Formulations of Zinc Diethyldithiocarbamate for Colorectal Cancer Treatment”, British Journal of Pharmacy 7(2). doi: https://doi.org/10.5920/bjpharm.1148