This research aimed to integrate an established signal quantification technique with immunocapture microneedle arrays (MNAs) to improve biomarker-based disease diagnostics in the skin. In this miniaturized sandwich enzyme-linked immunosorbent assay (ELISA) platform, the capture antibody was immobilised onto the surface of microneedles which, when inserted superficially into porcine skin, captured endogenous porcine immunoglobulin G (IgG) intradermally. Signal development was achieved by incubating the microneedles into-phenylenediamine (OPD) solution in a 384-well microplate and measuring the absorbance in a microplate spectrophotometer at 450 nm. This technique allows for rapid biomarker detection with high-throughput processing. Immunocapture microneedle devices such as these can be easily adapted for targeting different biomarkers or multiplexed leaving plenty of scope for future work and assay optimisation.
Microneedle Array (MNA); Point-of-Care Testing (POCT); Diagnostics; ELISA.
How to Cite
Dixon, R. V. & Lau, W. M. & Ng, K. W., (2022) “A Microneedle-Microplate Platform to Detect Biomarkers in the Skin”, British Journal of Pharmacy 7(2). doi: https://doi.org/10.5920/bjpharm.1164