Abstract
Physiologically based pharmacokinetic (PBPK) modelling is a powerful alternative for paediatric clinical trials. Paediatric PBPK models require data on intestinal drug metabolising enzymes and transporter (DMET)-protein abundances, however only limited data is available. This is the first study to report paediatric duodenal DMET protein quantification using a QconCAT approach. Thirty-six paediatric intestinal biopsies have been obtained from which the total mucosal protein was extracted. Proteins were digested to peptides using FASP and peptide levels were quantified using LC-MS/MS. Preliminary results provide some insight on the effect of age on duodenal protein abundance.
Keywords
Paediatrics, Proteomics, PBPK modelling, Biopharmaceutics
How to Cite
Goelen, J., Horniblow, R. D. & Batchelor, H., (2022) “Method development for quantification of DMET-proteins in the paediatric intestinal tract via LC-MS/MS using a QconCAT technique”, British Journal of Pharmacy 7(2). doi: https://doi.org/10.5920/bjpharm.1166
Funding
- Certara Simcyp
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