Compatibility of Caco-2 cells with Simulated Intestinal Fluid-Predicting Permeability

Abstract

The Caco-2 cell model is considered the gold standard when analysing drug permeability and risk-assessing bioavailability. The use of Simulated Intestinal Fluid (SIF) as a tool in the Caco-2 models is beneficial as this generates a more physiologically relevant model.  Existing work using biorelevant media in Caco-2 cells has not fully captured the large-scale variability that can exist within the GI fluids. Furthermore, there is some evidence that the composition of biorelevant fluids can disrupt the Caco-2 monolayer, particularly when high levels of bile and cholesterol are present in these media. Therefore, understanding the compatibility of the simulated intestinal media with the Caco-2 cells is essential to determine its use as transport media in a Caco-2 assay.

Keywords

Permeability, Simulated Intestinal fluid, Caco-2, Trans epithelial Resistance

How to Cite

Campbell, E. S., Batchelor, H., Butler, J. M., Khadra, I., Minstry, N. & Stamatopoulos, K., (2023) ‚ÄúCompatibility of Caco-2 cells with Simulated Intestinal Fluid-Predicting Permeability‚ÄĚ, British Journal of Pharmacy 8(2). doi: https://doi.org/10.5920/bjpharm.1403

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Authors

Erin Seonaid Campbell (University of Strathclyde)
Hannah Batchelor (Strathclyde)
James Matthew Butler
Ibrahim Khadra (University of Strathclyde)
Nena Minstry
Konstantinos Stamatopoulos (GlaxoSmithKline)

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Creative Commons Attribution 4.0

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This article has been peer reviewed.

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