The influence of oxygen and pressure on keratinocytes

Abstract

Thisstudy aims to elucidate the respective effects of normobaric hyperoxygenationand hyperbaric pressurisation on key re-epithelialisation processes in woundhealing. Cultured human keratinocytes exposed to intermittent normobaric hyperoxygenationexhibited enhanced cellularmigration marked by a significant decline in E-cadherin expression. Keratinocyteproliferation, cellular metabolic activity, as well as IL-6 and IL-8 releasewere also significantly reduced. These changes were not observed with hyperbaricpressurisation alone. Moreover, cellular differentiation was not altered undernormobaric hyperoxygenation or hyperbaric pressurisation. Thus, we concludethat hyperoxygenation differentially modulates key cellular processes in re-epithelialisation.Oxygenation, but not pressurisation, appears to be the predominant factor modulatingkeratinocyte migration and proliferation. These findings argue for an alternativetreatment modality to hyperbaric oxygenation for wound healing, focused on enhancingtissue oxygenation without administering hyperbaric pressures.

Keywords

wounds, hyperbaric oxygen, proliferation, re-epithelialisation

How to Cite

Williams A. , Obeid K. A. , Ng K. W. & Lau W. M. (2019) “The influence of oxygen and pressure on keratinocytes”, British Journal of Pharmacy. 4(1). doi: https://doi.org/10.5920/bjpharm.598

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Authors

Adrian Williams (University of Reading)
Kawa Ahmad Obeid (Sulaimani University)

Keng Wooi Ng (Newcastle University)

Wing Man Lau (Newcastle University)

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Licence

Creative Commons Attribution 4.0

Peer Review

This article has been peer reviewed.

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